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spatial gene expression profiling xenium in situ technology  (10X Genomics)

 
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    10X Genomics spatial gene expression profiling xenium in situ technology
    Spatial Gene Expression Profiling Xenium In Situ Technology, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene-expression+profiles/pm42288489-252-0-9
    Average 86 stars, based on 1 article reviews
    spatial gene expression profiling xenium in situ technology - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Hybridization:

    Article Title: Tumor sialylation regulates G-CSF stability and promotes neutrophil-mediated immunosuppression in breast cancer.
    Article Snippet: For each sample, one million cells were fixed for 22 hours at 4°C, quenched and stored at -80°C according to 10X genomic Fixation of Cells & Nuclei for Chromium Fixed RNA profiling (CG000478) using the Chromium Next GEM Single Cell Fixed RNA Sample preparation kit (PN-1000414, 10X Genomics, Pleasanton, CA). .. 250,000 cells per sample were used for probe hybridization using the Chromium Fixed RNA Kit, Mouse Transcriptome, 4rxn x 4BC (PN-1000496, 10X Genomics), pooled at equal numbers and washed following the Pooled Wash Workflow following the Chromium Fixed RNA Profiling Reagent kit protocol (CG000527, 10X Genomics). .. GEMs were generated on Chromium X (10X Genomics) with a target of 10,000 cells recovered and libraries prepared according to the manufacturer instructions (CG000527, 10X Genomics).

    Microscopy:

    Article Title: A phenotype-to-mechanism framework links phenome-wide comorbidity architecture to molecular mechanisms and therapeutic discovery in complex diseases
    Article Snippet: .. Further, Visium spatial transcriptome (10X Genomics) and immunofluorescent (IF) microscopy were performed to confirm their differential expression with spatial information at the RNA and protein level, respectively ( , and ). ..

    Quantitative Proteomics:

    Article Title: A phenotype-to-mechanism framework links phenome-wide comorbidity architecture to molecular mechanisms and therapeutic discovery in complex diseases
    Article Snippet: .. Further, Visium spatial transcriptome (10X Genomics) and immunofluorescent (IF) microscopy were performed to confirm their differential expression with spatial information at the RNA and protein level, respectively ( , and ). ..

    Generated:

    Article Title: DGAT: a dual-graph attention network for inferring spatial protein landscapes from transcriptomics.
    Article Snippet: .. Spatial transcriptomic libraries were generated with the Visium v2 Human Transcriptome gene expression kit (10x Genomics: 1000443). .. Five-micron FFPE sections were placed on Superfrost Plus slides (FisherScientific) and processed through the 10x Visium v2 for FFPE protocol according to the manufacturer’s instructions (CG000518, CG000520, CG000495).

    Transcriptome Wide Gene Expression:

    Article Title: DGAT: a dual-graph attention network for inferring spatial protein landscapes from transcriptomics.
    Article Snippet: .. Spatial transcriptomic libraries were generated with the Visium v2 Human Transcriptome gene expression kit (10x Genomics: 1000443). .. Five-micron FFPE sections were placed on Superfrost Plus slides (FisherScientific) and processed through the 10x Visium v2 for FFPE protocol according to the manufacturer’s instructions (CG000518, CG000520, CG000495).

    Sequencing:

    Article Title: Multiomic Analysis Reveals an IFN-driven Cellular Landscape Effectively Targeted by Ruxolitinib in Hailey-Hailey Disease
    Article Snippet: Libraries were sequenced on Illumina NovaSeq 6000 using S2 flow cell with a 28-10-10-90 cycle configuration (paired-end: R1 = 28 bp, i7 = 10 bp, i5 = 10 bp, R2 = 90 bp). .. Sequencing reads were demultiplexed and aligned to the human reference transcriptome (GRCh38-2020-A from 10x Genomics). .. Libraries were sequenced on Illumina NovaSeq 6000 using S2 flow cell with a 28-10-10-90 cycle configuration (paired-end: R1 = 28 bp, i7 = 10 bp, i5 = 10 bp, R2 = 90 bp).

    Single Cell:

    Article Title: Mapping neuro-vascular unit communications reveals distinct angiogenic programs across developing mouse brain regions.
    Article Snippet: .. Multiplexed libraries were prepared using the Chromium Next GEM Single Cell Fixed RNA Kit, Mouse Transcriptome, 4rxns x 4 BC (# 1000496, 10x Genomics; Pleasanton, CA, USA) AR TI CL E IN P RE SS according to the manufacturer’s instructions. .. After a PBS step washing, the myelin and the cell debris were separated from the pelleted cells by using the debris removal solution (Miltenyi Biotec, 130-109-398) and the cells were washed with PBS before proceeding to the red blood cell lysis (Miltenyi Biotec, 130-094-183).



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